To further standardize the application of robotic assisted single-port surgery in gynecology and enhance its operational safety and clinical outcomes, this expert consensus systematically summarizes the current technological status, indications, contradiction and operational protocols. In recent years, with advancements in endoscopic technology, robot-assisted laparoendoscopic single-site surgery (R-LESS) has significantly improved operational flexibility, precision, and surgeon comfort in gynecological procedures within confined spaces. The consensus elaborates on the technical features and clinical applications of multi-arm and single-arm robotic systems (such as da Vinci Si/Xi/SP, and domestically developed Shurui SR-ENS-600 and Jingfeng SP1000), defines the indications and contraindications including adnexal surgery, hysterectomy, and early-stage malignant tumor surgery, and standardizes key aspects of preoperative assessment, patient preparation, surgical steps, and complication management. Finally, it outlines future directions for R-LESS in AI-assisted planning, 5G remote operation, force feedback technology, and standardized training.