To report 1 case in China of a 5G-based remote robot-assisted laparoscopic surgery for endometrial cancer. A 49-year-old female patient presented with postmenopausal vaginal bleeding and discharge for over a month and was diagnosed preoperatively with endometrioid carcinoma (FIGO stage I) and uterine leiomyoma. After multidisciplinary evaluation, she underwent remote robot-assisted laparoscopic total hysterectomy, bilateral salpingo-oophorectomy, pelvic lymphadenectomy, pelvic adhesiolysis, and high ligation of ovarian vessels. The surgery was performed at the People′s Hospital of Xinjiang Uygur Autonomous Region by surgeons from the First Affiliated Hospital of Nanchang University, using a domestic four-arm laparoscopic robotic system remotely operated via a 5G wireless network and client equipment. The connection between the surgeon′s console and the operative platform was established through a public 5G network. During the procedure, the average air interface latency was 125 ms with a packet loss rate of 0.00%, and both signal coverage and retention ratio reached 100%. The surgery lasted 3 hours with approximately 100 ml of blood loss, and no intraoperative network or robotic malfunctions occurred. The patient recovered well, resumed oral intake and passed flatus on postoperative day 1, had the urinary catheter removed on day 2, and was discharged on day 3. Final pathology confirmed endometrioid adenocarcinoma. Follow-up showed satisfactory incision healing with no discomfort. This case demonstrates the preliminary feasibility and safety of 5G-based remote robot-assisted surgery for gynecologic malignancies in remote regions.